EpiPlex™ Duo-Mod™ Service
Detect, locate, and quantify m6A and inosine RNA modifications plus RNA expression, in one report.
Why Choose the EpiPlex™ Duo-Mod™ Service?
Turnkey Solution
End-to-end data delivery
We handle the complete workflow from RNA extraction, library prep through sequencing and analysis. Results are returned as an interactive EpiScout HTML report with downloadable BAM, BED, FASTQ, vcf, tsv, files, and publication-ready figures.
Multi-modification Analysis
Three RNA modifications in one report.
Get m6A and inosine mapping from the same samples, in addition to RNA seq. Correlate changes between modification types and find out which genes are up or downregulated in test and control conditions, and how the gene expression levels relate to RNA modification status. Identify equally expressed yet differentially modified genes to understand how the epitranscriptome modulates the proteome.
Relative Quantitation
Compare relative RNA modification levels.
Only a subset of RNA transcripts of a given gene contains RNA modifications to fine-tune function. The EpiPlex assay includes spike-in controls as normalization standards to infer the modification stoichiometry of each gene. Normalizing the assay signals in this way also reduces assay variability and enables data comparisons across operators and experiment batches.
Low RNA Input
High performance with a broad RNA input range
Tissue biopsies often contain less than 500 ng of total RNA, challenging many RNA sequencing methods. With the EpiPlex Duo-Mod Service, you can obtain exceptional data quality with as little as 20 ng poly(A)-selected RNA or 250 ng total RNA.