Detect more sites. Explore your data.
Understand the biology.
EpiScout delivers sensitive m6A, Ino, and Ψ analysis in one interactive report. Explore study summaries, QC, peak-level results, filterable volcano plots, and biological pathway enrichment, all in a single shareable file.
Everything you need to understand a study
See study quality, significant RNA modification changes, and biological pathways at a glance.
From sample comparisons to volcano plot in one click
See significant modification changes for every comparison, broken down by modification type. Click any row to open the corresponding interactive volcano plot, already filtered to that comparison.
Connect RNA modification changes to biological pathways
Gene Ontology (GO) Biological Process enrichment is calculated for each comparison and modification type, with fold enrichment and false discovery rate (FDR) reported for every significant process. Quickly identify biological themes associated with differential RNA modification, from immune response and development to metabolism.
Compare RNA modification profiles across libraries
The ternary plot shows the relative m6A, Ino, and Ψ composition of each library, making it easy to compare modification profiles across samples. Toggle between unique fragments, raw fragments, and detected peaks to view the data from different perspectives.
Distinguish RNA modification changes from expression changes
See whether significant events reflect modification enrichment, changes in transcription, or both. Stacked bars summarize enriched-only, expression-only, co-changing, and opposite-direction events for each comparison and modification type. Hover over any segment to see exact counts and percentages.
Identify QC issues and investigate them in one click
EpiScout summarizes QC warnings across the study and shows the number of affected samples. Click any flag to jump directly to the corresponding QC metric and see the data behind the warning.
Sortable tables. Expandable sections. CSV export on every table.
Read QC, peak-calling metrics, gene counts, and motif figures are organized into collapsible sections. Sort any column header, expand what you need, download the rest.
Explore differential RNA modification interactively
Move from tens of thousands of modification sites to the ones that matter most.
The interactive volcano plot updates in real time as you change comparisons, modification types,
and peak-region filters.
Find the modification sites you care about
Switch between comparisons and modification types, then filter by peak region—including DRACH motifs, exons, and introns—to narrow the volcano plot in real time. Combine multiple filters to define exactly the subset you want, then export the corresponding genes and results as a CSV.
From volcano plot to full event detail in one click
Click any point to see the gene, modification fold-change, enrichment signal, and reference RNA signal for each group. Open the Evidence tab for complete event metadata, or adjust plot labels in Display—all without leaving the volcano plot.
One file. Four tabs. Complete picture.
Overview
Study summary, QC status, modification fingerprints, signal landscape, and biological themes with direct links to detailed results.
Quality Control
Per-sample read, peak, and spike-in QC, with recommended-range warnings and one-click access to the underlying data.
Results
Peak-calling metrics, gene counts, motif enrichment, and metagene distributions in sortable tables with CSV export
Comparisons
Interactive volcano plots filterable by comparison, modification, peak region, strand, and signal pattern, with full event details available on click.
Hand your team a report they can actually use
No installs, no re-running scripts. Send a file and every collaborator gets the same interactive dashboard that is filterable, sortable, and tied back to raw data.