New! EpiPlex Duo-Mod Service End-to-end m6A and inosine profiling

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EpiPlex™ Platform Kit for m6A & Inosine Detection with RNA Expression

for highly reproducible, correlative data in a single reaction

m6A and inosine detection

The kit enables detection and relative quantification of m6A and inosine in RNA species ≥100 nucleotides. NGS library prep reagents for m6A and inosine detection plus RNA expression; UDI primers and EpiScout Compute Token are purchased separately.

8- or 24-sample workflow

Reagents for 8 or 24 samples in a 1-day workflow. Each sample is split into an enrichment reaction and a solution control, resulting in two sequencing libraries per sample.

Magnetic beads and standards

Magnetic beads simplify solution exchanges and enable multi-channel pipetting for ease of operation and throughput. Included RNA modification standards maximize robustness, serve as in-line quality controls, and enable relative quantification.

Validated sample types

Performance validated with DNase-treated, purified RNA from cell lines, tissue, and blood. User guides for total or poly(A)-selected RNA workflows are available.

Complete Bundles Available

EpiPlex RNA Mod Encoding Kit Starter Bundles (8 or 24 samples) include the kit, EpiPlex UDI Primer Kit, and EpiScout Analysis Software for a complete solution from library prep to epitranscriptomic analysis.

Built for real-world samples

Designed for challenging clinical and translational RNA, including FFPE, liquid biopsy, and low-input samples. The EpiPlex platform was built to generate high quality libraries from partially degraded RNA.

Product Details

Kit size
8- and 24-sample kits; higher quantities available upon request. Starter Bundles (kit + UDI + EpiScout Compute Token) in 8 or 24 samples.
Analysis
EpiScout™ Analysis Software via DNAnexus or docker; requires purchase of an EpiScout Compute Token for DNAnexus.
Required equipment
No specialized capital equipment. Compatible with short-read NGS sequencers and workflows.
Workflow duration
3 hour hands-on time; 7-hour total workflow.
Sample input
20 ng mRNA or 250 ng total RNA recommended; as little as 5 ng mRNA or 50 ng total RNA possible. Detects RNA species >100 nucleotides.
Sample type
Purified RNA from blood, cell lines, and frozen & FFPE tissues.
Genomes supported
Human, rat, and mouse as default; many more available upon request.
Sequencing requirements
25 million paired-end reads per library recommended; meaningful data with as little as 10M reads per library.
Documentation
See links in the sidebar →

EpiScout™ Analysis Software

RNA modification and RNA expression analysis for all scientists

Seamless data processing — Upload sequencing data and receive a comprehensive results report in a hands-off workflow.

Machine learning–trained peak calling — Accurate results with custom peak-calling algorithms.

Cloud app — User-friendly cloud app via DNAnexus.

Ready to order?

Use the pricing table above to add kits or bundles to your cart. Need a custom configuration or bulk quote? Contact us.

Pricing & Add to Cart

Prefer turnkey profiling?

EpiPlex™ Services—Uni-Mod, Duo-Mod, and Tri-Mod—offer send-sample RNA modification profiling with the same EpiScout™ analysis and quality.

View EpiPlex Services