Now available: EpiPlex Uni-, Duo-, and Tri-Mod Services. Profile m6A, inosine, and pseudouridine alongside gene expression.

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EpiPlex™ Platform Kit for m6A & Inosine Detection with RNA Expression

for highly reproducible, correlative data in a single reaction

m6A and inosine detection

The kit enables detection and relative quantification of m6A and inosine in RNA species ≥100 nucleotides. NGS library prep reagents for m6A and inosine detection plus RNA expression; UDI primers are purchased separately. Data analysis is included with each kit using the EpiScout™ Analysis Software.

8- or 24-sample workflow

Reagents for 8 or 24 samples in a 1-day workflow. Each sample is split into an enrichment reaction and a solution control, resulting in two sequencing libraries per sample.

Magnetic beads and standards

Magnetic beads simplify solution exchanges and enable multi-channel pipetting for ease of operation and throughput. Included RNA modification standards maximize robustness, serve as in-line quality controls, and enable relative quantification.

Validated sample types

Performance validated with DNase-treated, purified RNA from cell lines, tissue, and blood. User guides for total or poly(A)-selected RNA workflows are available.

Complete Solution

The EpiPlex RNA Mod Encoding Kits (8 or 24 samples) include EpiScout™ analysis on our cloud portal. Upload your sequencing data and explore m6A, inosine, and gene expression results with interactive visualizations.

Built for real-world samples

Designed for challenging clinical and translational RNA, including FFPE, liquid biopsy, and low-input samples. The EpiPlex platform was built to generate high quality libraries from partially degraded RNA.

Product Details

Kit size
8- and 24-sample kits; higher quantities available upon request.
Analysis
EpiScout™ Analysis Software on our cloud portal or using a docker image. Upload your sequencing data, run the analysis in a few clicks, and explore results in interactive visualizations, with no coding or bioinformatics experience required.
Required equipment
No specialized capital equipment. Compatible with short-read NGS sequencers and workflows.
Workflow duration
3 hour hands-on time; 7-hour total workflow.
Sample input
20 ng mRNA or 250 ng total RNA recommended; as little as 5 ng mRNA or 50 ng total RNA possible. Detects RNA species >100 nucleotides.
Sample type
Purified RNA from blood, cell lines, and frozen & FFPE tissues.
Genomes supported
Human, rat, and mouse as default; many more available upon request.
Sequencing requirements
25 million paired-end reads per library recommended.
Documentation
See links in the sidebar →

EpiScout™ Analysis Software

RNA modification and RNA expression analysis for all scientists

Seamless data processing — Upload sequencing data and receive a comprehensive results report in a hands-off workflow.

Machine learning–trained peak calling — Accurate results with custom peak-calling algorithms.

Cloud portal — Analyze your data and explore results in interactive visualizations on our secure cloud portal.

Ready to order?

Use the pricing table above to add kits or bundles to your cart. Need a custom configuration or bulk quote? Contact us.

Pricing & Add to Cart

Prefer turnkey profiling?

EpiPlex™ Services—Uni-Mod, Duo-Mod, and Tri-Mod—offer send-sample RNA modification profiling with the same EpiScout™ analysis and quality.

View EpiPlex Services